ISSN: 1405-888X ISSN-e: 2395-8723
Análisis del crecimiento y contenido de lípidos de la diatomea Caloneis amphisbaena var. subsalina aislada de la laguna costera “San José el Hueyate”, Chiapas, México
Caloneis amphisbaena var. subsalina, recolectada en la laguna costera "San José el Hueyate", Chiapas, México (fotografía tomada por Néstor Barrios Morales en el Centro de Investigación de los Sistemas Costeros y Continentales, Universidad Autónoma de Chiapas).
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Palabras clave

diatomea
costa de Chiapas
laguna costera
parámetros de crecimiento
triglicéridos

Cómo citar

de la Cruz-Armenta, M., Teco-Bravo, J. I., Tovar-Juárez, E., López-Arroyo, M. de los Ángeles, Estrada-Santos, Y., & Negrete-Moreno, P. M. (2026). Análisis del crecimiento y contenido de lípidos de la diatomea Caloneis amphisbaena var. subsalina aislada de la laguna costera “San José el Hueyate”, Chiapas, México. TIP Revista Especializada En Ciencias Químico-Biológicas, 29. https://doi.org/10.22201/fesz.23958723e.2026.786

Resumen

Las diatomeas producen lípidos que por sus características son viables para uso biotecnológico, sin embargo, el crecimiento y la producción de éstos metabolitos es taxonómicamente específico. En este estudio se aisló la diatomea Caloneis amphisbaena var. subsalina de la laguna costera “San José el Hueyate”, Edo. de Chiapas, México, se evaluó su crecimiento, producción de biomasa, contenido de lípidos totales y triglicéridos. Este microorganismo no tuvo un período de adaptación y alcanzó la fase estacionaria en seis días, su tasa y velocidad de crecimiento, así como tiempo de duplicación fue de 0.94 d−1; 2,436,111 células mL−1 d−1 y 0.73 d, respectivamente. La producción de biomasa de 3,456 mg L−1 y 576 mg L−1 d−1, el contenido de lípidos totales de 501.33 mg L−1 (83.56 mg L−1 d−1 y 13.52% de biomasa) y el de triglicéridos 273.82 mg L−1 (45.64 mg L−1 d−1, 65.94% de los lípidos totales y 8.14% de biomasa). Los resultados de este estudio sugieren que C. amphisbaena var. subsalina tiene potencial para la producción de biomasa y lípidos con fines biotecnológicos, comparable con los de diatomeas modelo y con otros aislamientos obtenidos de diversas latitudes.

https://doi.org/10.22201/fesz.23958723e.2026.786
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Citas

Alishah-Aratboni, H., Rafiei, N., Garcia-Granados, R., Alemzadeh, A. & Morones-Ramírez, J. R. (2019). Biomass and lipid induction strategies in microalgae for biofuel production and other applications. Microbial Cell Factories, 18(1), 178. https://doi.org/10.1186/s12934-019-1228-4

Bastos, C. R. V., Maia, I. B., Pereira, H., Navalho, J. & Varela, J. C. S. (2022). Optimisation of Biomass Production and Nutritional Value of Two Marine Diatoms (Bacillariophyceae), Skeletonema costatum and Chaetoceros calcitrans. Biology, 11(4), 594. https://doi.org/10.3390/biology11040594

Bhattacharjya, R., Kiran Marella, T., Tiwari, A., Saxena, A., Kumar Singh, P. & Mishra, B. (2020). Bioprospecting of marine diatoms Thalassiosira, Skeletonema and Chaetoceros for lipids and other value-added products. Bioresource Technology, 318, 124073. https://doi.org/10.1016/j.biortech.2020.124073

Cañavate, J. P., van Bergeijk , S., González‒Ortegón, E. & Vílas, C. (2021). Contrasting fatty acids with other indicators to assess nutritional status of suspended particulate organic matter in a turbid estuary. Estuarine, Coastal and Shelf Science, 254, 107329. https://doi.org/10.1016/j.ecss.2021.107329.

Celekli, A., Toudjani, A., Gümüş, E. Y., Kayhan, S., Lekesiz, H. & Cetin, T. (2019). Determination of trophic weight and indicator values of diatoms in Turkish running waters for water quality assessment. Turkish Journal of Botany, 43(1), 90-101. https://doi.org/10.3906/bot-1704-40.

Cleve, P. T. (1894). Synopsis of the Naviculoid Diatoms: Presented to the R. Swedish Academy of Sciences May 10, 1893. Norstedt.

Cointet, E., Séverin, E., Couzinet-Mossion, A., Méléder, V., Gonçalves, O. & Wielgosz-Collin, G. (2021). Assessment of the lipid production potential of six benthic diatom species grown in airlift photobioreactors. Journal of Applied Phycology, 33(4), 2093-2103. https://doi.org/10.1007/s10811-021-02490-4

Cointet, E., Wielgosz-Collin, G., Méléder, V. & Gonçalves, O. (2019). Lipids in benthic diatoms: A new suitable screening procedure. Algal Research, 39, 101425. https://doi.org/10.1016/j.algal.2019.101425

Dhanker, R., Saxena, A., Tiwari, A., Singh, P. K., Patel, A. K., Dahms, H. U. & Parra-Saldívar, R. (2024). Towards sustainable diatom biorefinery: Recent trends in cultivation and applications. Bioresource Technology, 391, 129905. https://doi.org/10.1016/j.biortech.2023.129905

Díaz-Ruiz, S., Aguirre-Leon, A. & Cano-Quiroga, E. (2006). Evaluación ecológica de las comunidades de peces en dos sistema lagunares estuarinos del sur de Chiapas, México. Hidrobiológica, 16(2), 197-210.

Duarte, B., Feijão, E., Goessling, J. W., Caçador, I. & Matos, A. R. (2021). Pigment and Fatty Acid Production under Different Light Qualities in the Diatom Phaeodactylum tricornutum. Applied Sciences, 11(6), 2550. https://doi.org/10.3390/app11062550

Eberle, M. E. (2016). Recent diatoms reported from the central United States: register of taxa and synonyms. State Biological Survey of Kanas, (Report Number 77). https://scholars.fhsu.edu/biology_facpubs/5.

Gillard, J. T. F., Hernandez, A. L., Contreras, J. A., Francis, I. M. & Cabrales, L. (2021). Potential for Biomass Production and Remediation by Cultivation of the Marine Model Diatom Phaeodactylum tricornutum in Oil Field Produced Wastewater Media. Water, 13(19), 2700. https://doi.org/10.3390/w13192700

Govindan, N., Maniam, G., Ab. Rahim, M., Sulaiman, A., Ajit, A., Chatsungnoen, T. & Chisti, Y. (2021). Production of Renewable Lipids by the Diatom Amphora copulata. Fermentation, 7(1), 37. https://doi.org/10.3390/fermentation7010037

Groß, E., Boersma, M. & Meunier, C. L. (2021). Environmental impacts on single-cell variation within a ubiquitous diatom: The role of growth rate. PLOS ONE, 16(5), e0251213. https://doi.org/10.1371/journal.pone.0251213

Grubišić, M., Šantek, B., Kuzmić, M., Čož-Rakovac, R. & Ivančić Šantek, M. (2024). Enhancement of Biomass Production of Diatom Nitzschia sp. S5 through Optimisation of Growth Medium Composition and Fed-Batch Cultivation. Marine Drugs, 22(1), 46. https://doi.org/10.3390/md22010046

Grubišić, M., Šantek, B., Zorić, Z., Čošić, Z., Vrana, I., Gašparović, B., Čož-Rakovac, R. & Ivančić Šantek, M. (2022). Bioprospecting of Microalgae Isolated from the Adriatic Sea: Characterization of Biomass, Pigment, Lipid and Fatty Acid Composition, and Antioxidant and Antimicrobial Activity. Molecules, 27(4), 1248. https://doi.org/10.3390/molecules27041248

Heckman, C. W. (1985). The development of vertical migration patterns in the sediments of estuaries as a strategy for algae to resist drift with tidal currents. Internationale Revue der gesamten Hydrobiologie und Hydrographie, 70(1), 151-164. https://doi.org/10.1002/iroh.19850700112.

Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M. & Darzins, A. (2008). Microalgal triacylglycerols as feedstocks for biofuel production: Perspectives and advances. The Plant Journal, 54(4), 621-639. https://doi.org/10.1111/j.1365-313X.2008.03492.x

INE (Instituto Nacional de Ecología) (1999). Programa de Manejo de la Reserva de la Biosfera de La Encrucijada. SEMARNAP, México. https://www.conanp.gob.mx/datos_ abiertos/DGCD/50.pdf

Indrayani, I., Moheimani, N. R., de Boer, K., Bahri, P. A. & Borowitzka, M. A. (2020a). Temperature and salinity effects on growth and fatty acid composition of a halophilic diatom, Amphora sp. MUR258 (Bacillariophyceae). Journal of Applied Phycology, 32, 977-987.

Indrayani, I., Haslianti, H., Asmariani, A., Muskita, W. & Balubi, A. M. (2020b). Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas Journal of Biological Diversity, 21(4), 1498-1503. https://doi.org/10.13057/biodiv/d210430

Jasprica, N. & Hafner, D. (2005). Taxonomic composition and seasonality of diatoms in three Dinaric karstic lakes in Croatia. Limnologica, 35(4), 304-319. https://doi.org/10.1016/j.limno.2005.08.003.

Kaleli, A. & Akçaalan, R. (2021). Checklist of marine diatoms from the Turkish coastal waters with updated nomenclature. Aquatic Research, 4(1), 88-115. https://doi.org/10.3153/AR21008

Khan, M. J., Bawra, N., Verma, A., Kumar, V., Pugazhendhi, A., Joshi, K. B. & Vinayak, V. (2021). Cultivation of diatom Pinnularia saprophila for lipid production: A comparison of methods for harvesting the lipid from the cells. Bioresource Technology, 319, 124129. https://doi.org/10.1016/j.biortech.2020.124129

Lang, I., Hodac, L., Friedl, T. & Feussner, I. (2011). Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biology, 11(1), 124. https://doi.org/10.1186/1471-2229-11-124.

Lara Villa, M. A., Moreno Ruiz, J. L. & Amaro Mauricio, E. J. (1996). Fitoplancton: conceptos básicos y técnicas de laboratorio. Universidad Autónoma Metropolitana, Unidad Iztapalapa, Ciudad de México, México

Lemley, D. A., Adams, J. B. & Bate, G. C. (2016). A review of microalgae as indicators in South African estuaries. South African Journal of Botany, 107, 12‒20. https://doi.org/10.1016/j.sajb.2016.04.008.

Li, Y., Deng, L., Walker, E. J. L., Karas, B. J. & Mock, T. (2025). Genetic engineering in diatoms: advances and prospects. The Plant Journal, 121(6), e70102. https://doi.org/10.1111/tpj.70102.

Lucas, C., Widdows, J., Brinsley, M., Salkeld, P. & Herman, P. (2000). Benthic-pelagic exchange of microalgae at a tidal flat. 1. Pigment analysis. Marine Ecology Progress Series, 196, 59-73. https://doi.org/10.3354/meps196059

Marella, T. K. & Tiwari, A. (2020). Marine diatom Thalassiosira weissflogii based biorefinery for co-production of eicosapentaenoic acid and fucoxanthin. Bioresource Technology, 307, 123245. https://doi.org/10.1016/j.biortech.2020.123245

Madigan, M. T., Martinko, J. M. & Parker, J. (2004). Brock, biología de los microorganismos. (p. 1011). Pearson, USA.

Miho, A. & Witkowski, A. (2005). Diatom (Bacillariophyta) Flora of Albanian Coastal Wetlands Taxonomy and Ecology: A Review. Proceedings of the California Academy of Sciences, 56(12), 129-145.

Montes-Cartas, C. G., Castillo-Argüero, S. & López-Portillo, J. (1999). Distribución del manglar en cuatro sistemas lagunares en la costa de Chiapas, México. Botanical Sciences, 64, 25-34. https://doi.org/10.17129/botsci.1579

Morales, M., Aflalo, C. & Bernard, O. (2021). Microalgal lipids: A review of lipids potential and quantification for 95 phytoplankton species. Biomass and Bioenergy, 150, 106108. https://doi.org/10.1016/j.biombioe.2021.106108

Moreno-Ruíz, J. L., Licea, S. & Santoyo, H. (1996). Diatomeas del golfo de California. Universidad Autónoma de Baja California Sur, SEP-FOMES/PROMARCO. pp. 1-273.

Nascimento, I. A., Marques, S. S. I., Cabanelas, I. T. D., Pereira, S. A., Druzian, J. I., De Souza, C. O., Vich, D. V., De Carvalho, G. C. & Nascimento, M. A. (2013). Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria. BioEnergy Research, 6(1), 1-13. https://doi.org/10.1007/s12155-012-9222-2

Nieri, P., Carpi, S., Esposito, R., Costantini, M. & Zupo, V. (2023). Bioactive Molecules from Marine Diatoms and Their Value for the Nutraceutical Industry. Nutrients, 15(2), 464. https://doi.org/10.3390/nu15020464

Oliver, A., Podell, S., Pinowska, A., Traller, J. C., Smith, S. R., McClure, R., Beliaev, A., Bohutskyi, P., Hill, E. A., Rabines, A., Zheng, H., Zeigler Allen, L., Kuo, A., Grigoriev, I. V., Allen, A. E., Hazlebeck, D. & Allen, E. E. (2021). Diploid genomic architecture of Nitzschia inconspicua, an elite biomass production diatom. Scientific Reports, 11(1), 15592. https://doi.org/10.1038/s41598-021-95106-3

Pérez-Jiménez, G. M., Rivas-Acuña, Ma. G., León Álvarez, D., Campos Campos, B. & Quiroz-González, N. (2020). Macroalgas de la laguna «El Carmen», Tabasco, México. Acta Botanica Mexicana, 127 (e1606), 1-12. https://doi.org/10.21829/abm127.2020.1606

Qin, L., Wang, Z., Sun, Y., Shu, Q., Feng, P., Zhu, L., Xu, J. & Yuan, Z. (2016). Microalgae consortia cultivation in dairy wastewater to improve the potential of nutrient removal and biodiesel feedstock production. Environmental Science and Pollution Research, 23(9), 8379-8387. https://doi.org/10.1007/s11356-015-6004-3

Sacristán-de Alva, M., Luna-Pabello, V. M., Orta-Ledesma, M. T. & Cruz-Gómez, M. J. (2018). Carbon, nitrogen, and phosphorus removal, and lipid production by three saline microalgae grown in synthetic wastewater irradiated with different photon fluxes. Algal Research, 34, 97-103. https://doi.org/10.1016/j.algal.2018.07.006.

Salama, E.-S., Kurade, M. B., Abou-Shanab, R. A. I., El-Dalatony, M. M., Yang, I.-S., Min, B. & Jeon, B.-H. (2017). Recent progress in microalgal biomass production coupled with wastewater treatment for biofuel generation. Renewable and Sustainable Energy Reviews, 79, 1189-1211. https://doi.org/10.1016/j.rser.2017.05.091

Sarayloo, E., Tardu, M., Unlu, Y. S., Simsek, S., Cevahir, G., Erkey, C. & Kavakli, I. H. (2017). Understanding lipid metabolism in high-lipid-producing Chlorella vulgaris mutants at the genome-wide level. Algal Research, 28, 244-252. https://doi.org/10.1016/j.algal.2017.11.009

Shanks, A. L. & McCulloch, A. (2003). Fortnightly periodicity in the abundance of diatom and dinoflagellate taxa at a coastal study site. Journal of Experimental Marine Biology and Ecology, 296(1), 113-126. https://doi.org/10.1016/S0022-0981(03)00320-4

Sharma, N., Simon, D. P., Diaz-Garza, A. M., Fantino, E., Messaabi, A., Meddeb-Mouelhi, F., Germain, H. & Desgagné-Penix, I. (2021). Diatoms biotechnology: various industrial applications for a greener tomorrow. Frontiers in Marine Science, 8, 636613. https://doi.org/10.3389/fmars.2021.636613.

Shokravi, Z., Shokravi, H., Chyuan, O. H., Lau, W. J., Koloor, S. S. R., Petrů, M. & Ismail, A. F. (2020). Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review. Sustainability, 12(21), 9083. https://doi.org/10.3390/su12219083

Suparmaniam, U., Lam, M. K., Lim, J. W., Yusup, S., Tan, I. S., Lau, S. Y. & Kachhwaha, S. S. (2023). Influence of environmental stress on microalgae growth and lipid profile: a systematic review. Phytochemistry Reviews, 22(4), 879-901. https://doi.org/10.1007/s11101-022-09810-7

Tanaka, T., Yoneda, K. & Maeda, Y. (2022). Lipid metabolism in diatoms. In Falciatore & Mock (Eds). The Molecular Life of Diatoms (pp. 493-527) Paris: Springer International Publishing. https://doi.org/10.1007/978-3-030-92499-7.

Teco-Bravo, J. I., Barahona-Pérez, L. F., Reyes-Sosa, C. F., Ku-González, Á. F., Herrera-Valencia, V. A. & Peraza-Echeverria, S. (2019). Enhanced production of triacylglycerols and polyunsaturated fatty acids in novel acid-tolerant mutants of the green microalga Chlorella saccharophila. Bioprocess and Biosystems Engineering, 42(10), 1561-1571. https://doi.org/10.1007/s00449-019-02153-2

Teco-Bravo, J. I., Tovar-Juárez, E., Estrada-Santos, Y., Negrete-Moreno, P. M. & López-Arroyo, M. de los Á. (2021a). Aislamiento y cinética de crecimiento de microalgas de la laguna costera «Pampa el Cabildo», Puerto Madero, Chiapas, México. La Ciencia Aplicada en Chiapas, 7, 29-39.

Teco-Bravo, J. I., Barahona-Pérez, L. F., Peraza-Echeverria, S., Baas-Espínola, F. M., Reyes-Sosa, C. F. & Herrera-Valencia, V. A. (2021b). Lipid profiles of acid-tolerant mutants of the green microalga Chlorella saccharophila reveal hydrocarbons and high-value lipids with potential industrial applications. Bioresource Technology Reports, 13, 100636. https://doi.org/10.1016/j.biteb.2021.100636.

Varona-Cordero, F. & Gutiérrez-Mendieta, F. J. (2006). Composición estacional del fitoplancton de dos lagunas costeras del Pacífico tropical. Hidrobiológica, 16(2), 159-174.

Varona-Cordero, F., Gutierrez-Mendieta, F. J. & Meave Del Castillo, M. E. (2010). Phytoplankton assemblages in two compartmentalized coastal tropical lagoons (Carretas-Pereyra and Chantuto-Panzacola, Mexico). Journal of Plankton Research, 32(9), 1283-1299. https://doi.org/10.1093/plankt/fbq043

Vázquez, G., Aké-Castillo, J. A. & Orduña Medrano, R. E. (2021). Phytoplankton Catalog of Coastal Systems of the Gulf of Mexico and Caribbean Sea (1.a ed.). EPOMEX-UAC. https://doi.org/10.26359/epomex.cemie102021

Velázquez-Sánchez, I. G., Tovar Juárez, E., Estrada Santos, Y., Negrete Moreno, P. M., Herrera Valencia, V. A., Peraza Echeverria, S. & Teco Bravo, J. I. (2023). Nitrogen and phosphorus removal coupled to CO2 fixation by two green microalgae, Chlorella sp. and Quadrigula sp., native to the coast of Chiapas, Mexico. Mexican Journal of Biotechnology, 8(4), 68-89. https://doi.org/10.29267/mxjb.2023.8.4.68

Widjaja, A., Chien, C.-C. & Ju, Y.-H. (2009). Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. Journal of the Taiwan Institute of Chemical Engineers, 40(1), 13-20. https://doi.org/10.1016/j.jtice.2008.07.007

Yi, Z., Xu, M., Di, X., Brynjolfsson, S. & Fu, W. (2017). Exploring valuable lipids in diatoms. Frontiers in Marine Science, 4, 17. https://doi.org/10.3389/fmars.2017.00017.

Zhu, Z., Sun, J., Fa, Y., Liu, X. & Lindblad, P. (2022). Enhancing microalgal lipid accumulation for biofuel production. Frontiers in Microbiology, 13, 1024441. https://doi.org/10.3389/fmicb.2022.1024441

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