Keywords
vineyard pruning
fungal cultivation
gene expression
How to Cite
Abstract
The Ganoderma genus is recognized for its high content of bioactive compounds, including triterpenoids. The biosynthesis of these metabolites occurs via the mevalonate pathway and is regulated by the expression of key genes such as fps, sqs, and ls. This study analyzed the early expression of these genes in Ganoderma lucidum and G. mexicanum under static liquid culture. Vineyard pruning extracts prepared with different solvents were used to induce triterpenoid production. Gene expression was evaluated in mycelium cultured at 0, 0.5, 1, and 24 h with elicitor (ht=treatment) or alcohol (hc=control), along with colorimetric quantification of triterpenoids. In G. lucidum, sqs reached its highest expression at 0.5 ht (20.56-fold increase), while fps was higher at 0 h, and ls remained repressed. In G. mexicanum, sqs showed its highest values at 24 ht (11.47-fold increase) and at 1 hc (8.03-fold increase), while fps and ls were more expressed at 24ht and 1hc. The quantification of triterpenoids partially coincided with expression patterns. These results suggest that vineyard pruning extracts effectively induce metabolite production without genetic modifications, highlighting the utility of optimizing culture conditions.
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