Abstract
Heavy metal pollution represents a significant environmental threat in water bodies, particularly in regions with intense mining and agricultural activities such as Chihuahua, Mexico. This study evaluated the ability of a native strain of Chlorella vulgaris to tolerate and remove lead (Pb) and hexavalent chromium [Cr(VI)] under laboratory conditions. The microalga was cultivated in Miracle-Gro medium under controlled photoperiod, temperature, and aeration. The minimum inhibitory concentration (MIC), growth kinetics, and metal removal efficiency were assessed at 5 and 50 mg/L. Results showed that Chlorella vulgaris tolerates low concentrations of Pb and removes over 90% within the first 48 hours. In contrast, Cr(VI) exhibited significant toxicity, drastically reducing biomass even at 5 mg/L. Microscopic observations revealed cellular damage associated with Cr(VI) exposure. It is concluded that Chlorella vulgaris has high potential for bioremediation of lead-contaminated water, although critical limitations are identified for hexavalent chromium. This study supports the development of sustainable, microalgae-based technologies for wastewater treatment in northern Mexico.
References
Bajguz, A. (2011). Suppression of Chlorella vulgaris growth by cadmium, lead, and copper stress and its restoration by endogenous brassinolide. Archives of Environmental Contamination and Toxicology, 60(3), 406-416. https://link.springer.com/article/10.1007/s00244-010-9551-0?utm_source=chatgpt.com
Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M. R. & Sadeghi, M. (2021). Toxic mechanisms of five heavy metals: mercury, lead, chromium, cadmium, and arsenic. Frontiers in Pharmacology, 12, 643972. https://doi.org/10.3389/fphar.2021.643972
Balzano, S., Sardo, A., Blasio, M., Chahine, T. B., Dell’Anno, F., Sansone, C. & Brunet, C. (2020). Microalgal metallothioneins and phytochelatins and their potential use in bioremediation. Frontiers in Microbiology, 11, 517. https://doi.org/10.3389/fmicb.2020.00517
Cervantes, K. F. H. (2019). Aislamiento e identificación de microalgas y evaluación de parámetros en dinámica de crecimiento en fijación de CO2. Tesis de Maestría en Estudios y Gestión Ambiental. Universidad Autónoma de Ciudad Juárez. 70.
Chanquia, S. N., Vernet, G. & Kara, S. (2022). Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives. Engineering in Life Sciences, 22(12), 712-724. https://doi.org/10.1002/elsc.202100070
Chakraborty, S., Talukdar, A., Dey, S. & Bhattacharya, S. (2025). Role of fungi, bacteria and microalgae in bioremediation of emerging pollutants with special reference to pesticides, heavy metals and pharmaceuticals. Discover Environment, 3(1), 91. https://doi.org/10.1007/s44274-025-00217-7
Chugh, M., Kumar, L., Shah, M. P. & Bharadvaja, N. (2022). Algal Bioremediation of heavy metals: An insight into removal mechanisms, recovery of by-products, challenges, and future opportunities. Energy Nexus, 7, 100129. https://doi.org/10.1016/j.nexus.2022.100129
Fitri, W. E., Putra, A. & Febria, F. A. (2024). Removal of Heavy Metals Using Chlorella vulgaris: A Review. Journal Katalisator, 9(1), 148-162. http://doi.org/10.22216/jk.v5i2.5717
Gallegos, W., Vega, M. & Noriega, P. (2012). Espectroscopía de absorción atómica con llama y su aplicación para la determinación de plomo y control de productos cosméticos. La Granja, 15(1), 19-29. https://doi.org/10.17163/lgr.n15.2012.02
Garbisu, C., Amézaga, I. & Alkorta, I. (2002). Biorremediación y ecología. Ecosistemas, 11(3), 1-2. http//www.aeet.org/ecosistemas/023/opinion1.htm
Hedayatkhah, A., Cretoiu, M. S., Emtiazi, G., Stal, L. J. & Bolhuis, H. (2018). Bioremediation of chromium contaminated water by diatoms with concomitant lipid accumulation for biofuel production. Journal of Environmental Management, 227, 313-320. https://doi.org/10.1016/j.jenvman.2018.09.011
Hernández-Peña, C. C., Lares-Villa, F., Santos-Villalobos, S. D. L., Estrada-Alvarado, M. I., Cruz-Soto, A., Flores-Tavizon, E. & Soto-Padilla, M. Y. (2021). Reduction in concentration of chromium (VI) by Lysinibacillus macroides isolated from sediments of the Chapala Lake, Mexico. Anais da Academia Brasileira de Ciências, 93(2), e20190144. https://doi.org/10.1590/0001-3765202120190144
Inthorn, D., Sidtitoon, N., Silapanuntakul, S. & Incharoensakdi, A. (2002). Sorption of mercury, cadmium and lead by microalgae. Sci. Asia, 28(3), 253-261. https://doi.org/10.2306/scienceasia1513-1874.2002.28.253
Jacome-Pilco, C., Ballesteros, C., Rea, E. & Cayambe, L. M. R. (2021). Microalgas en el tratamiento de aguas residuales generadas en industrias de curtiembres. Ciencia y Tecnología, 14(2), 47-55. https://doi.org/10.18779/cyt.v14i2.502
Kumar, K. S., Dahms, H. U., Won, E. J., Lee, J. S. & Shin, K. H. (2015). Microalgae a promising tool for heavy metal remediation. Ecotoxicology and Environmental Safety, 113, 329-352. https://doi.org/10.1016/j.ecoenv.2014.12.019
Kusuma, H. S., Illiyanasafa, N., Jaya, D. E. C., Darmokoesoemo, H. & Putra, N. R. (2024). Utilization of the microalga Chlorella vulgaris for mercury bioremediation from wastewater and biomass production. Sustainable Chemistry and Pharmacy, 37, 101346. https://doi.org/10.1016/j.scp.2023.101346
Kyratzopoulou, E., Kyzaki, N., Malletzidou, L., Nerantzis, E. & Kazakis, N. A. (2025). The Efficiency of Chlorella vulgaris in Heavy Metal Removal: A Comparative Study of Mono-and Multi-Component Metal Systems. Clean Technologies, 7(2), 35. https://doi.org/10.3390/cleantechnol7020035
Lee, L., Hsu, C. Y. & Yen, H. W. (2017). The effects of hydraulic retention time (HRT) on chromium (VI) reduction using autotrophic cultivation of Chlorella vulgaris. Bioprocess and Biosystems Engineering, 40(12), 1725-1731. https://doi.org/10.1007/s00449-017-1827-6
Leong, Y. K. & Chang, J. S. (2020). Bioremediation of heavy metals using microalgae: Recent advances and mechanisms. Bioresource technology, 303, 122886. https://doi.org/10.1016/j.biortech.2020.122886
Lu, M. M., Gao, F., Li, C. & Yang, H. L. (2021). Response of microalgae Chlorella vulgaris to Cr stress and continuous Cr removal in a membrane photobioreactor. Chemosphere, 262, 128422. https://doi.org/10.1016/j.chemosphere.2020.128422
Mahlangu, D., Mphahlele, K., De Paola, F. & Mthombeni, N. H. (2024). Microalgae mediated biosorption for effective heavy metals removal from wastewater: A review. Water, 16(5), 718. https://doi.org/10.3390/w16050718
Mustafa, S., Bhatti, H. N., Maqbool, M. & Iqbal, M. (2021). Microalgae biosorption, bioaccumulation and biodegradation efficiency for the remediation of wastewater and carbon dioxide mitigation: Prospects, challenges and opportunities. Journal of Water Process Engineering, 41, 102009. https://doi.org/10.1016/j.jwpe.2021.102009
Papu, N. H. & Lingfa, P. (2018, April). Isolation, biomass estimation and characterization of the biofuel potential of diatom Navicula Sphaerophora. In AIP Conference Proceedings (Vol. 1952, No. 1, p. 020060). AIP Publishing LLC. https://doi.org/10.1063/1.5032022
Razzak, S. A., Bahar, K., Islam, K. O., Haniffa, A. K., Faruque, M. O., Hossain, S. Z. & Hossain, M. M. (2024). Microalgae cultivation in photobioreactors: Sustainable solutions for a greener future. Green Chemical Engineering, 5(4), 418-439. https://doi.org/10.1016/j.gce.2023.10.004
Reyes, Y., Vergara, I., Torres, O., Lagos, M. D. & Jimenez, E. E. G. (2016). Contaminación por metales pesados: Implicaciones en salud, ambiente y seguridad alimentaria. Ingeniería Investigación y Desarrollo: I2+ D, 16(2), 66-77. https://core.ac.uk/download/pdf/386133717.pdf
Rezaei, M., Pourang, N. & Moradi, A. M. (2022). Removal of lead from aqueous solutions using three biosorbents of aquatic origin with the emphasis on the affective factors. Scientific Reports, 12(1), 751. https://doi.org/10.1038/s41598-021-04744-0
Rodríguez Heredia, D. (2017). Intoxicación ocupacional por metales pesados. Medisan, 21(12), 3372-3385. http://scielo.sld.cu/pdf/san/v21n12/san122112.pdf
Sánchez-Bayo, A., Morales, V., Rodríguez, R., Vicente, G. & Bautista, L. F. (2020). Cultivation of microalgae and cyanobacteria: Effect of operating conditions on growth and biomass composition. Molecules, 25(12), 2834. https://doi.org/10.3390/molecules25122834
Singh, V., Singh, N., Rai, S. N., Kumar, A., Singh, A. K., Singh, M. P., Sahoo, A., Shekhar, S., Vamanu, E. & Mishra, V. (2023). Heavy metal contamination in the aquatic ecosystem: toxicity and its remediation using eco-friendly approaches. Toxics, 11(2), 147. https://doi.org/10.3390/toxics11020147
Spennati, E., Casazza, A. A., Perego, P., Solisio, C., Busca, G. & Converti, A. (2019). Microalgae growth in winery wastewater under dark conditions. Chemical Engineering Transactions, 74, 1471–1476. https://doi.org/10.3303/CET1974246
Teoh, M. L. & Wong, S. W. (2018). Influence of lead on growth and physiological characteristics of a freshwater green alga Chlorella sp. Malaysian Journal of Science, 37(2), 82-93. http://dx.doi.org/10.22452/mjs.vol37no2.1
Travieso, L., Canizares, R. O., Borja, R., Benitez, F., Dominguez, A. R., Dupeyrón y, R. & Valiente, V. (1999). Heavy metal removal by microalgae. Bulletin of Environmental Contamination and Toxicology, 62(2), 144-151.
Zhang, W., Xiong, B., Chen, L., Lin, K., Cui, X., Bi, H., Guo, M. & Wang, W. (2013). Toxicity assessment of Chlorella vulgaris and Chlorella protothecoides following exposure to Pb (II). Environmental Toxicology and Pharmacology, 36(1), 51-57. https://doi.org/10.1016/j.etap.2013.03.003
Zhang, P., Yang, M., Lan, J., Huang, Y., Zhang, J., Huang, S., Yang, Y. & Ru, J. (2023). Water quality degradation due to heavy metal contamination: Health impacts and eco-friendly approaches for heavy metal remediation. Toxics, 11(10), 828. https://doi.org/10.3390/toxics11100828
Zhao, D., Cheah, W. Y., Lai, S. H., Ng, E. P., Khoo, K. S., Show, P. L. & Ling, T. C. (2023). Symbiosis of microalgae and bacteria consortium for heavy metal remediation in wastewater. Journal of Environmental Chemical Engineering, 11(3), 109943. https://doi.org/10.1016/j.jece.2023.109943
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